Microparticle sorting apparatus and delay time determination method

ABSTRACT

A microparticle sorting apparatus includes a detection unit which detects microparticles flowing through a flow path; an imaging device which images a droplet containing the microparticles which is discharged from an orifice provided on an edge portion of the flow path; a charge unit which applies a charge to the droplets; and a control unit which determines a delay time as from a time that the microparticles are detected by the detection unit to the time at which a number of bright spots in a standard region, which is set beforehand, of image information imaged by the imaging device reaches the maximum, making it possible for the charge unit to apply a charge to the microparticles once the delay time has lapsed after the microparticles are detected by the detection unit.

RELATED APPLICATIONS

This application is a continuation of and claims the benefit under 35U.S.C. § 120 of U.S. patent application Ser. No. 15/093,879, titled“MICROPARTICLE SORTING APPARATUS AND DELAY TIME DETERMINATION METHOD,”filed on Apr. 8, 2016 which is a divisional of and claims the benefitunder 35 U.S.C. § 120 of U.S. patent application Ser. No. 13/788,075,titled “MICROPARTICLE SORTING APPARATUS AND DELAY TIME DETERMINATIONMETHOD,” filed on Mar. 7, 2013, which claims the benefit under 35 U.S.C.§ 119 of Japanese Patent Application JP 2012-080192, filed in theJapanese Patent Office on Mar. 30, 2012, each of which is herebyincorporated by reference in its entirety.

BACKGROUND

The present technology relates to a microparticle sorting apparatus anda delay time determination method in the microparticle sortingapparatus. More specifically, the present technology relates to amicroparticle sorting apparatus or the like which automaticallydetermines the delay time.

In the related art, there is a microparticle sorting apparatus (forexample, a flow cytometer) which optically, electrically, ormagnetically detects the characteristics of microparticles such ascells, then separates and collects only the microparticles which havepredetermined characteristics.

In cell separation in a flow cytometer, first, a droplet stream (alaminar flow of a sample fluid containing cells and a sheath fluid) isgenerated from an orifice formed in the flow cell, the fluid stream ismade into droplets by applying oscillation to the orifice, and a chargeis applied to the droplets. Furthermore, the movement direction of thedroplets containing the cells discharged from the orifice iselectrically controlled and the target cells having the desiredcharacteristics and the other non-target cells are collected in separatecollection containers.

For example, Japanese Unexamined Patent Application Publication No.2010-190680 discloses, as a microchip-type flow cytometer, “amicroparticle sorting apparatus including: a microchip on which a flowpath through which a fluid containing microparticles flows, and anorifice which discharges a fluid which flows through a flow path to aspace outside of the chip are installed; an oscillating element formaking a fluid into droplets in the orifice and discharging them; anelectrical charging means for applying a charge to the dischargeddroplets; an optical detection means for detecting the opticalcharacteristics of the microparticles flowing though the flow path; anelectrode couple installed opposing one another to interpose the movingdroplets; and two or more containers which collect the droplets whichpassed between the opposing electrodes”.

In addition, Japanese Unexamined Patent Application Publication(Translation of PCT Application) No. 2007-532874 discloses a method inwhich control is performed on the operation of a flow cytometer which iscapable of confirming whether or not the droplets have been sorted intoan intended flow path by disposing auxiliary lighting and a detectionunit in the position at which the droplets break off from the fluid(hereinafter, referred to as the break-off point). By ascertaining thebreak-off point in this manner, it is possible to ascertain the delaytime from when the microparticles, which are cells or the like, aredetected until the droplets containing the cells or the like reach thebreak-off point, and it is possible to apply a charge to the dropletscontaining the microparticles which are detected based on the delaytime.

SUMMARY

However, the break-off point fluctuates according to the dischargeconditions of the droplets and the like, and therefore the delay timealso fluctuates. In addition, it is difficult to sufficiently ascertainan accurate timing to apply a charge to the droplets containing themicroparticles by only ascertaining the break-off point. Therefore, thecorrect charge is applied to the droplets which contain themicroparticles, but in the end, methods have mostly been adopted inwhich the user visually discriminates whether the droplets have beenallotted to the desired collection container or not by observing thedroplets, to which a charge is applied, on a preparation. Such methodsdemanded that the user have a mastery of the technology, and there areproblems with the reliability and stability.

Therefore, it is desirable to provide a microparticle sorting apparatuswhich is capable of automatically, simply, and accurately applying acharge to droplets.

According to an embodiment of the present technology, there is provideda microparticle sorting device including a detection unit which detectsmicroparticles flowing through a flow path; an imaging device whichimages droplets containing the microparticles which are discharged froman orifice provided on an edge portion of the flow path; a charge unitwhich applies a charge to the droplets; and a control unit whichdetermines a delay time as from a time that the microparticles aredetected by the detection unit to the time at which the number of brightspots in a standard region, which is set beforehand, of imageinformation imaged by the imaging device reaches the maximum, making itpossible for the charge unit to apply a charge to the microparticlesonce the delay time has lapsed after the microparticles are detected bythe detection unit.

According to the microparticle sorting apparatus, it is possible toautomatically determine the delay time based on the number of brightspots within the standard region without asking for the user to performsetting.

In the microparticle sorting apparatus, the imaging device may imageimages of a plurality of the droplets at a plurality of different times,and the control unit may preliminarily determine the delay time usingthe time from when the microparticles are detected by the detection unitto the time at which the number of bright spots within the standardregion, which is calculated by comparing the image information of theplurality of droplets imaged by the imaging device, reaches a maximum asa provisional delay time.

In the microparticle sorting apparatus, the imaging device may image theimages of the plurality of droplets within a shorter time than adischarge interval time of each of the droplets once the provisionaldelay time has elapsed from the time at which the microparticles aredetected by the detection unit, and the control unit may update theprovisional delay time and determine the delay time by referring toadjacent information relating to the number of the bright spots in atleast one of two comparison regions that are adjacent to the standardregion in relation to a discharge direction of the droplets in the imageinformation.

In the microparticle sorting apparatus, of the two comparison regions, afirst comparison region and a second comparison region may be arrangedin order in a discharge direction of the droplets, and the control unitmay calculate the time at which the number of bright spots reaches apredetermined percentage of the maximum value in the second comparisonregion in a process where the number of bright spots increases towardthe time at which the number of bright spots reaches the maximum in thesecond comparison region, and may determine the time to be the time atwhich the number of bright spots within the standard region reaches amaximum.

In the microparticle sorting apparatus, of the two comparison regions, afirst comparison region and a second comparison region may be arrangedin order in a discharge direction of the droplets, and the control unitmay calculate the first time at which the number of bright spots reachesa predetermined percentage of the maximum value in the first comparisonregion in a process where the number of bright spots decreases from thetime at which the number of bright spots reaches the maximum in thefirst comparison region, may calculate the second time at which thenumber of bright spots reaches a predetermined percentage of the maximumvalue in the second comparison region in a process where the number ofbright spots increases toward the time at which the number of brightspots reaches the maximum in the second comparison region, and maydetermine the time in which the number of bright spots within thestandard region reaches a maximum as (the first time+the second time)/2.

In the microparticle sorting apparatus, the control unit may adjust adischarge frequency of the droplets, and determines the optimaldischarge frequency of the droplets to be the discharge frequency atwhich the break-off point, where the droplets start forming in thedischarge direction of the droplets, is closest to the orifice.

In addition, the microparticle sorting apparatus may further include apair of deflection plates disposed opposite one another to interpose thedroplets imaged by the imaging device, which change a progressiondirection of the droplets using an electrical force which acts betweenthe deflection plates and the charge.

In addition, the microparticle sorting apparatus may also be amicrochip-type flow cytometer in which the orifice and the flow path areprovided in the microchip.

Furthermore, the term “delay time” here refers to the delay time fromthe time at which the microparticles are detected by the detection unitto when the droplets are formed from the fluid containing themicroparticles. In other words, “delay time” refers to the necessarytime from the time that the microparticles are detected by the detectionunit to when the droplets containing the microparticles have a chargeapplied thereto. In the present technology, this refers to the durationof from the time at which the microparticles are detected by thedetection unit, to the time at which the number of bright spots withinthe standard region, which is set beforehand, in the image informationimaged by the imaging device reaches the maximum.

In addition, the term “provisional delay time” refers to a provisionaldelay time until the delay time is determined. More specifically,“provisional delay time” refers to the time from when the microparticlesare detected by the detection unit to the time at which the number ofbright spots in the standard region, which is calculated by comparingthe plurality of items of image information of the droplets imaged bythe imaging device, reaches the maximum. In addition, an example of theimaging device includes a droplet camera or the like.

In addition, according to another embodiment of the present technology,there is provided a delay time determination method including a processof causing a microparticle sorting device to detect microparticlesflowing through the flow path, causing a microparticle sorting device toimage the droplets containing the microparticles discharged from theorifice provided on the edge portion of the flow path, and causing amicroparticle sorting device to determine the delay time as from thetime that the microparticles are detected until the time at which thenumber of bright spots within the standard region, which is setbeforehand, in the image information of the imaged droplets reaches themaximum.

In addition, in the method, a process may also be included which imagesthe images of each of a plurality of droplets at a plurality of times,performs preliminarily determination of the delay time using the periodfrom when the microparticles are detected until the time at which thenumber of bright spots in the standard region, which is calculated bycomparing the image information of each of the plurality of dropletswhich are imaged, reaches the maximum as the provisional delay time.

In addition, in the method, a process may also be included which imagesthe images of the plurality of droplets within a shorter time than adischarge interval time of each of the droplets once the provisionaldelay time has elapsed from the time at which the microparticles aredetected, and updates the provisional delay time and determines thedelay time by referring to adjacent information relating to the numberof the bright spots in at least one of two comparison regions that areadjacent to the standard region in relation to a discharge direction ofthe droplets in the image information.

In addition, in the method, a process may also be included in which, ofthe two comparison regions, a first comparison region and a secondcomparison region are arranged in order in a discharge direction of thedroplets, the first time at which the number of bright spots reaches apredetermined percentage of the maximum value in the second comparisonregion in a process where the number of bright spots increases towardthe time at which the number of bright spots reaches the maximum in thesecond comparison region is calculated, and the second time to be thetime at which the number of bright spots within the standard regionreaches the maximum is determined.

In addition, in the method, a process may also be included in which, ofthe two comparison regions, a first comparison region and a secondcomparison region are arranged in order in a discharge direction of thedroplets, the time at which the number of bright spots reaches apredetermined percentage of the maximum value in the first comparisonregion in a process where the number of bright spots decreases from thetime at which the number of bright spots reaches the maximum in thefirst comparison region is calculated, the time at which the number ofbright spots reaches a predetermined percentage of the maximum value inthe second comparison region in a process where the number of brightspots increases toward the time at which the number of bright spotsreaches the maximum in the second comparison region is calculated, andthe time in which the number of bright spots within the standard regionreaches a maximum is determined as (the first time+the second time)/2.

In the present technology, the term “microparticle” widely includesorganism related microparticles such as cells, microorganisms andliposomes, or synthetic particles such as latex particles, gel particlesand particles for industrial use.

The organism related microparticles include chromosomes, liposomes,mitochondria, organelles and the like which various types of cellcontain. The cells include animal cells (hematopoietic cells and thelike) and plant cells. Microorganisms include bacteria such asEscherichia coli, viruses such as the tobacco mosaic virus, and fungisuch as yeast. Furthermore, for the organism related microparticles,nucleic acid and protein, and organism related polymers such ascomplexes thereof may also be included. In addition, particles forindustrial use may also be, for example, organic or inorganic polymericmaterials, metals, and the like. The organic polymeric materials includepolystyrene, divinylbenzene styrene, polymethyl methacrylate, and thelike. The inorganic polymer materials include glass, silica, magneticmaterials, and the like. The metals include metal colloid, aluminum, andthe like. It is normal for the shape of these microparticles to begenerally spherical, however, they may also be non-spherical, and thesize, mass, and the like thereof is not particularly limited.

It is desirable to provide a microparticle sorting apparatus which iscapable of automatically, simply, and accurately applying a charge todroplets.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a schematic view for illustrating the configuration of thesorting system of a microparticle sorting apparatus (a flow cytometer)according to an embodiment of the present technology which is configuredas a microchip-type flow cytometer;

FIGS. 2A and 2B are schematic views for illustrating an example of amicrochip which may be installed in the flow cytometer;

FIGS. 3A to 3C are schematic views for illustrating the configuration ofan orifice of the microchip;

FIG. 4 is a flow diagram for illustrating the method of determining thedelay time in the flow cytometer;

FIGS. 5A to 5D are explanatory diagrams, which are photographs showingexamples of images of a droplet imaged by a droplet camera of the flowcytometer, for illustrating the provisional delay time determinationstep;

FIGS. 6A to 6C are explanatory diagrams for illustrating the delay timedetermination step in the flow cytometer;

FIGS. 7A to 7C are explanatory diagrams, which are photograph showingexamples of images of the droplets imaged by the droplet camera of theflow cytometer, for illustrating the delay time determination step;

FIG. 8 is an explanatory diagram, which is a graph in which thehorizontal axis is the phase, and the vertical axis is the number ofbright spots of the images of the droplets imaged by the droplet cameraof the flow cytometer, for illustrating an example of the delay timedetermination step (the first delay time determination method);

FIG. 9 is an explanatory diagram, which is a graph in which thehorizontal axis is the phase, and the vertical axis is the number ofbright spots of the images of the droplets imaged by the droplet cameraof the flow cytometer, for illustrating an example of the delay timedetermination step (the first delay time determination method);

FIG. 10 is an explanatory diagram, which is a graph in which thehorizontal axis is the phase, and the vertical axis is the number ofbright spots of the images of the droplets imaged by the droplet cameraof the flow cytometer, for illustrating an example of the delay timedetermination step (the second delay time determination method);

FIG. 11 is an explanatory diagram, which is a graph in which thehorizontal axis is the phase, and the vertical axis is the number ofbright spots of the images of the droplets imaged by the droplet cameraof the flow cytometer, for illustrating an example of the delay timedetermination step (the second delay time determination method); and

FIG. 12 is a flow diagram for illustrating the method of sorting themicroparticles (the microparticle sorting step) in the flow cytometer.

DETAILED DESCRIPTION OF EMBODIMENTS

Hereafter, description will be given of favorable embodiments forrealizing the present technology with reference to the drawings.Furthermore, the embodiments described below represent an example of arepresentative embodiment of the present technology, and the scope ofthe present technology is not to be interpreted narrowly according tothis example. The description will be given in the following order.

1. Apparatus Configuration of Microparticle Sorting Apparatus accordingto Present Technology

1-1 Charge Unit

1-2 Microchip

1-3 Detection Unit

1-4 Droplet Camera

1-5 Deflection Plate

1-6 Collection Container

1-7 Control Unit or Similar

2. Delay Time Determination Method in Microparticle Sorting Apparatusaccording to Present Technology

2-1 Microparticle Detection Step S₁

2-2 Droplet Discharge Step S₂

2-3 Droplet Imaging Step S₃

2-4 Discharge Frequency Determination Step S₄

2-5 Provisional Delay Time Determination Step S₅

2-6 Delay Time Determination Step S₆

2-6-1 First Delay Time Determination Method

2-6-2 Second Delay Time Determination Method

2-7 Microparticle Sorting Step S₇

2-7-1 Microparticle Detection Step S₇₁

2-7-2 Droplet Discharge and Charge Application Step S₇₂

1. Apparatus Configuration of Microparticle Sorting Apparatus Accordingto Present Technology

FIG. 1 is a schematic view for illustrating the configuration of thesorting system of the microparticle sorting apparatus 1 (hereinafteralso referred to as “the flow cytometer 1”) according to an embodimentof the present technology which is configured as a microchip-type flowcytometer.

1-1 Charge Unit

The flow cytometer 1 is provided with a charge unit 11 which applies acharge to the droplets discharged from the orifice 21 formed on themicrochip 2. The charging of the droplets is performed by electrodes 12,which are electrically connected to the charge unit 11 and inserted intoa sample inlet 23 provided on the microchip 2. Furthermore, it issufficient for the electrodes 12 to be inserted to a location on themicrochip 2 so as to make electrical contact with the sample fluid orthe sheath fluid which is pumped down the flow path.

In the flow cytometer 1, it is possible for the charge unit 11 to chargethe droplets containing the microparticles once the delay time haselapsed after the microparticles contained in the sample fluid aredetected by a detection unit 3 described below. Here, the term “delaytime” refers to the delay time from the time that the microparticles aredetected by the detection unit 3 to when droplets are formed from thefluid containing the microparticles. In other words, “delay time” refersto the necessary time from the time that the microparticles are detectedby the detection unit 3 to when the droplets containing themicroparticles have a charge applied thereto by the charge unit 11. Inthe present technology, the term “delay time” refers to the duration offrom the time at which the microparticles are detected by the detectionunit 3, to the time at which the number of bright spots within thestandard region, which is set beforehand, in the image informationimaged by the droplet camera 4 described below reaches a maximum.

1-2 Microchip

FIGS. 2A and 2B and FIGS. 3A to 3C show an example of the microchip 2which may be installed in the flow cytometer 1.

FIG. 2A shows a schematic view of the upper surface, and FIG. 2B shows across-sectional schematic view corresponding to the line IIB-IIB in FIG.2A. In addition, FIGS. 3A to 3C schematically illustrate theconfiguration of the orifice 21 of the microchip 2, FIG. 3A shows anupper surface schematic view, FIG. 3B shows a cross-section schematicview, and FIG. 3C shows a front surface schematic view. FIG. 3Bcorresponds to the cross section along the line IIIB-IIIB in FIG. 2A.

The microchip 2 is formed of substrate layers 2 a and 2 b which arebonded together to form a sample flow path 22. It is possible to performthe formation of the sample flow path 22 from the substrate layers 2 aand 2 b through injection molding of a thermoplastic resin using a metalmold. For the thermoplastic resin, plastics generally used in therelated art as microchip materials such as polycarbonate, polymethylmethacrylate resin (PMMA), cyclic polyolefin, polyethylene, polystyrene,polypropylene and polymethyl disilazane (PDMS) may be adopted.

The sample fluid is introduced to the sample inlet 23 from the fluiddelivery connector portion, merges with the sheath fluid which isintroduced from the fluid delivery connector portion to a sheath inlet24, and is delivered through the sample flow path 22. The sheath fluidintroduced from the sheath inlet 24, after being split into twodirections and delivered, in the merging portion in which the sheathfluid merges with the sample fluid introduced from the sample inlet 23,the sheath fluid merges with the sample fluid so as to interpose thesample fluid from two directions. Therefore, in the merging portion, inthe center of the sheath fluid laminar flow, a three-dimensional laminarflow in which the sample laminar flow is positioned is formed.

Reference numeral 25 represents a suction flow path for applying anegative pressure to the sample flow path 22 when clogging or bubblesoccur in the sample flow path 22, which temporarily causes the flow toflow backward in order to resolve the clogging or bubbles. On one end ofa suction flow path 25, a suction outlet 251 connected to a negativepressure source such as a vacuum pump via the liquid delivery connectorportion is formed, and the other end is connected to the sample flowpath 22 in a communication hole 252.

In the three-dimensional laminar flow, the laminar flow width is limitedin a limiter portion 261 (refer to FIGS. 2A and 2B) and 262 (refer toFIGS. 3A to 3C) formed such that the surface area of the vertical crosssection thereof in relation to the fluid delivery direction gets smallergradually or in stages from upstream in the fluid delivery direction todownstream. After this, the three-dimensional laminar flow becomes afluid stream (refer to FIG. 1) from the orifice 21 provided on one sideof the flow path, and is discharged. In FIG. 1, the discharge directionof the fluid stream from the orifice 21 is represented by the Y axispositive direction.

The connection portion to the orifice 21 of the sample flow path 22 is astraight portion 27, which is formed linearly. The straight portion 27functions such that the fluid stream from the orifice 21 is ejected in astraight line in the Y axis positive direction.

The fluid stream ejected from the orifice 21 is transformed intodroplets by the oscillation applied to the orifice 21 by a chipexcitation unit. The orifice 21 is open in the end face direction of thesubstrate layers 2 a and 2 b, and a notch portion 211 is providedbetween the opening position thereof and the substrate layer end face.The notch portion 211 is formed by notching the substrate layers 2 a and2 b between the opening portion of the orifice 21 and the substrate endface such that the diameter L of the notch portion 211 is larger thanthe opening diameter 1 of the orifice 21 (refer to FIG. 3C). It isdesirable to form the diameter L of the notch portion 211 two times orlarger than the opening diameter 1 of the orifice 21 so as not toobstruct the movement of the droplets discharged from the orifice 21.

1-3 Detection Unit

The reference numeral 3 in FIG. 1 represents the detection unit whichdetects the measurement target light emitted from the microparticlessuch as cells through the irradiation of a laser L1 emitted from a lightsource 31. The detection unit 3 performs characteristic detection of thecells between the limiter portion 261 (refer to FIGS. 2A and 2B) and thelimiter portion 262 (refer to FIGS. 3A to 3C) of the sample flow path22. The characteristic detection is not particularly limited, however,for example, in a case in which optical detection is used, the scatteredlight and the fluorescent light emitted from the cells due to theirradiation of laser L1 (refer to FIG. 1) in relation to the cells whichare fluid delivered arranged in a single row in the sample flow path 22in the center of the three-dimensional laminar flow, are detected by thedetection unit 3.

For the irradiation and detection of the light, in addition to the laserlight source, irradiation systems that condense and irradiate a laseronto the cells such as a condensing lens, a dichroic mirror or a bandpass filter may also be configured. The detection system is, forexample, configured by an area imaging device such as a PMT (photomultiplier tube), or a CCD or CMOS device.

The measurement target light detected by the detection system of thedetection unit 3 is light emitted from the cells due to the irradiationof the measurement light, and for example, may be scattered light,fluorescent light or the like such as forward scattered light, sidescattered light, Rayleigh scattering, or Mie scattering. Thesemeasurement target lights are converted into an electrical signal,output to a control unit 7, and utilized in the optical characteristicdiscrimination of the cells.

Furthermore, the detection unit 3 may also detect the characteristics ofthe cells magnetically or electrically. In this case, microelectrodesare disposed opposing one another in the sample flow path 22 of themicrochip 2, and the resistance value, the capacitance value, theinductance value, the impedance, the change value of the electric fieldbetween the electrodes, or the magnetization, the change in the magneticfield, or the like are measured.

1-4 Droplet Camera

The reference numeral 4 in FIG. 1 represents an example of the imagingdevice of the present technology, which is a droplet camera for imagingthe droplet D discharged from the orifice 21 of the microchip 2 such asa CCD camera, a CMOS sensor, or the like. The droplet camera 4 isdesigned such that it is possible to perform focus adjustment of theimage of the droplet D which is imaged. In the flow cytometer 1, thedroplets containing microparticles such as cells are irradiated by alaser L2 emitted from the light source 41, and the microparticles areexcited while the droplet D is imaged by the droplet camera 4, therebythe flow cytometer 1 is designed such that the user can confirm that themicroparticles are contained in the droplets from the display unit.

In addition, in the flow cytometer 1, due to the microchip beingexchanged for a new microchip, or the external environment (thetemperature and the like) changing, there are cases in which it isnecessary to change the droplet formation parameters (sheath pressure,droplet frequency, piezo drive pressure, and the like). In this case, itis necessary to adjust the time until the charge is applied to thedroplets containing the microparticles after the microparticles aredetected by the detection unit 3 (hereinafter, this time is alsoreferred to as the delay time). The droplet camera 4 functions in orderto image the droplet D, and also in order to make it possible for thecontrol unit 7 described below to determine the delay time.

More specifically, the droplet camera 4 is designed such that it ispossible to image a plurality of images of the droplet D at a pluralityof different times such that the control unit 7 described below maypreliminarily determine the provisional delay time as the delay time.Furthermore, the term “provisional delay time” refers to the duration offrom the time at which the microparticles are detected by the detectionunit 3, to the time at which the number of bright spots within thestandard region, which is calculated by comparing a plurality of itemsof image information of the droplets imaged by the droplet camera 4,reaches a maximum. In addition, the term “plurality of different times”,for example, refers to each time, the interval between which is the timeof the reciprocal of the frequency of the oscillation which anoscillating element 13 applies to the orifice 21 (in other words, thedischarge interval time of each of the droplets D).

In addition, in order for the control unit 7 to be able to update theprovisional delay time and determine the delay time, the droplet camera4 is designed to be able to image a plurality of images of the droplet Dwithin a predetermined time after the provisional delay time has elapsedfrom the time at which the microparticles are detected by the detectionunit 3. Furthermore, the term “predetermined time” refers to a timeshorter than the discharge interval time of each of the droplets D.

In addition, the droplet camera 4 is designed to be movable in thepositive direction or the negative direction along the Y axis such thatthe control unit 7 may determine the optimal discharge frequency of thedroplets D described below.

In addition, the images imaged by the droplet camera 4 are displayed onthe display unit such as a display, and are also used to allow the userto confirm the formation state of the droplet D (the size, shape,interval, and the like of the droplet) in the orifice 21.

1-5 Deflection Plate

The reference numerals 51 and 52 in FIG. 1 represent a pair ofdeflection plates which are disposed opposite one another to interposethe droplet D which is ejected from the orifice 21 and imaged by thedroplet camera 4. Deflection plates 51 and 52 are configured to containthe electrodes which control the movement direction of the dropletsdischarged from the orifice 21 using the electrical force on the chargeapplied to the droplets. In addition, the deflection plates 51 and 52also control the trajectory of the droplet D emitted from the orifice 21using the electrical force on the charge applied to the droplet D. InFIG. 1, the opposing direction of the deflection plates 51 and 52 isrepresented by the X axis direction.

1-6 Collection Container

In the flow cytometer 1, the droplet D is accepted by one of theplurality of collection containers 611, 612, 62, or 63 which aredisposed in a row in the opposing direction of the deflection plates 51and 52 (the X axis direction). The collection containers 611, 612, 62,or 63 may also be plastic tubes or glass tubes which are normally usedfor experiments. The number of the collection containers 611, 612, 62,or 63 is not particularly limited, however, here, a case in which fourare disposed is illustrated. The droplet D emitted from the orifice 21is guided into and collected in one of the four collection containers611, 612, 62, or 63 according to the presence or absence, oralternatively the magnitude of the electrical force between thedeflection plates 51 and 52.

The collection containers 611, 612, 62, and 63 are disposed in acontainer for use as the collection container (not shown) in anexchangeable manner. The container for use as the collection container(not shown) is disposed on the Z axis stage (not shown) configured to bemovable in the direction (the Z axis direction) orthogonal to thedischarge direction (the Y axis direction) of the droplet D from theorifice 21 and to the opposing direction (X axis direction) of thedeflection plates 51 and 52.

1-7 Control Unit or Similar

The flow cytometer 1, in addition to the configuration described above,is provided with a data analysis unit for characteristic discriminationof the cells or the like detected by the detection unit 3, a tankportion for retaining the sample fluid and the sheath fluid, the controlunit 7 for controlling each of the configurations described above, andthe like which an ordinary flow cytometer is provided with.

The control unit 7 may be configured by an ordinary computer providedwith a CPU, memory, a hard disk and the like, and on the hard disk isstored the OS, a program to execute each step relating to the delay timedetermination method described next, and the like.

2. Delay Time Determination Method in Microparticle Sorting ApparatusAccording to Present Technology

2-1 Microparticle Detection Step S₁

FIG. 4 is flow chart for illustrating the delay time determination stepin the flow cytometer 1. The delay time determination step includes theprocesses of “microparticle detection step S₁”, “droplet discharge stepS₂”, “droplet imaging step S₃”, “discharge frequency determination stepS₄”, “provisional delay time determination step S₅”, and “delay timedetermination step S₆”. In addition, a process of “microparticle sortingstep S₇” may also be executed after the delay time determination stepsdescribed above. Description will be given of each process below.

First, in the microparticle detection step S₁, the control unit 7outputs a signal to the fluid delivery connector portion and beginsfluid delivery of the sample fluid and the sheath fluid. Furthermore,the detection unit 3 detects the microparticles contained in the sampleat the sample flow path 22 by, for example, the irradiation of the laserL. Furthermore, the present step S₁ and the steps S₂ to S₆ describedbelow are a calibration process for determining the delay time from whenthe detection unit 3 detects the target cells or the like until thecharge unit 11 applies a charge to the droplets containing the cells orthe like. Therefore, it is preferable to use calibration beads such asparticles for industrial use, the shape and the like of which is clear,beforehand as the microparticles.

2-2 Droplet Discharge Step S₂

In the droplet discharge step S₂, the oscillating element 13 applies anoscillation to the orifice 21, the droplet D is discharged from theorifice 21, the droplets D is collected in the waste fluid inlet, and itis possible to dispose of the fluid (refer to FIG. 4).

2-3 Droplet Imaging Step S₃

In the droplet imaging step S₃, the control unit 7 outputs a signal tothe droplet camera 4, and the droplet camera 4 that received the signalimages the droplet D which is discharged and excited by the laser L1(refer to FIG. 4). The droplet camera 4 may image the images at aninterval that is the same as or shorter than the interval of the dropletclock described below.

At this time, for example, the control unit 7 may output a signal to thedroplet camera 4 and make the droplet camera 4 that received the signalmove in the X axis direction or the Y axis direction. Furthermore, thecontrol unit may perform focus adjustment in the Z axis direction in theimaging of the images of the droplet D by the droplet camera 4. Forexample, the control unit 7 may perform focus adjustment until thecontrast ratio in the image imaged by the droplet camera 4 falls withina predetermined range.

2-4 Discharge Frequency Determination Step S₄

In the discharge frequency determination step S₄, the control unit 7moves the droplet camera 4 to a predetermined position and adjusts thedischarge frequency of the droplets D based on the image informationimaged by the droplet camera 4 (refer to FIG. 4). The predeterminedposition described above is not particularly limited, however, it may bea position set beforehand according to the discharge conditions such asthe orifice diameter and the drive pressure.

Furthermore, the control unit 7 determines the optimal dischargefrequency of the droplets D to be the discharge frequency at which theposition where the droplets D start forming in the Y axis direction(hereinafter referred to as the break-off point) is closest to theorifice 21. Furthermore, the present step S₄ may also be executed afterthe step S₅ described below.

In this manner, in the flow cytometer 1, since the optimal dischargefrequency is determined by the control unit 7 based on the break-offpoint, it is possible to resolve the complication of the user settingthe droplet frequency manually.

2-5 Provisional Delay Time Determination Step S₅

In the provisional delay time determination step S₅, the control unit 7determines the provisional delay time of the droplet D by comparing theplurality of items of image information of the droplet D imaged by thedroplet camera 4 from the time at which the microparticles are detectedby the detection unit 3 (refer to FIG. 4).

The term “provisional delay time” here refers to the time which isprovisionally treated as the delay time by the present step S₅, which isthe period until the delay time is determined by the delay time step S₆described below. More specifically, the term “provisional delay time”refers to the duration of from the time at which the microparticles aredetected by the detection unit 3, to the time at which the number ofbright spots within the standard region described below, which iscalculated by comparing a plurality of items of image information of thedroplet D imaged by the droplet camera 4 at a plurality of differenttimes, reaches a maximum. Furthermore, the term “plurality of differenttimes” is not particularly limited, however, for example, refers to eachtime, the interval between which is the time of the reciprocal of thefrequency of the oscillation which the oscillating element 13 applies tothe orifice 21 (in other words, refers to the discharge interval time ofeach of the droplets D, and is referred to as the “droplet clock”hereinafter).

FIGS. 5A to 5D are photographs showing examples of images of a dropletimaged by the droplet camera 4 of the flow cytometer 1, and representthe images which are imaged at different times (refer to FIGS. 5A to5D). More specifically, FIGS. 5A to 5D are photographic views forillustrating which of the droplets the detected microparticles arecontained in when the droplet D imaged by the droplet camera 4 at thetime (T0) at which the microparticle is detected by the detection unit 3is set as the first droplet. Furthermore, each photographic view mayalso be a view in which a plurality of imaged images are integratedtogether.

In FIGS. 5A to 5D, the term “Section 1” refers to the standard regionset beforehand in the image P.

The control unit 7 compares the plurality of images of the droplet Dimaged by the droplet camera 4 at the interval of the droplet clock, andpreliminarily determines the time from T0 until when the number of thebright spots B within “Section 1” reaches a maximum as the provisionaldelay time. Furthermore, the term “bright spot” refers to pixels whichhave a higher brightness than a predetermined threshold in the image ofthe droplet D imaged by the droplet camera 4, and is an item of imageinformation of the microparticles contained in the excited droplet D,irradiated by the laser L2.

In FIGS. 5A to 5D, as an example of the present technology, the term“bright spot” refers to the images which are imaged by the dropletcamera 4 when the 30th to 33rd droplets are discharged, when the dropletD discharged from the orifice 21 at T0 and imaged by the droplet camera4 is set as the first droplet. For example, the 30th droplet is thephotographic view represented by N=30 (refer to FIG. 5A).

In the example shown in FIGS. 5A to 5D, the control unit 7 candiscriminate that the microparticles are contained in the 32nd dropletbased on the image information of N=32 (refer to FIG. 5C) in which thenumber of bright spots B within “Section 1” reaches a maximum. In otherwords, the control unit 7 compares the plurality of images of thedroplet D imaged by the droplet camera 4 at the interval of the dropletclock, and can preliminarily determine the delay time from the time thatthe microparticles are detected until the time that the 32nd droplet isdischarged as the provisional delay time.

In this manner, in the flow cytometer 1, it is possible to preliminarilydetermine the provisional delay time as the delay time by comparing thenumber of bright spots in the image information within “Section 1” inrelation to a plurality of different times. Therefore, it is possible tosimply, and accurately apply a charge to the droplets without demandingcomplicated operations of the user.

2-6 Delay Time Determination Step S₆

In the delay time determination step S₆ shown in FIG. 4, first, thecontrol unit 7 refers to the adjacent information described below basedon the plurality of images of the droplet D imaged by the droplet camera4 within a shorter time than the droplet clock after the provisionaldelay time has elapsed from T0. Furthermore, the control unit 7 updatesthe provisional delay time which is preliminarily determined by theprovisional delay time determination step S₅ described above, and maydetermine the delay time more precisely by referring to the adjacentinformation. The method is described below with reference to FIG. 6A toFIG. 11. Furthermore, the adjacent information is information related tothe number of bright spots in the two comparison regions of standardregions which are adjacent in relation to the discharge direction of thedroplets.

FIGS. 6A to 6C schematically show the transition from when themicroparticles are detected by the detection unit 3 to the droplet Dcontaining microparticles is imaged by the droplet camera 4.

FIG. 6A shows a graph of the droplet clock (Droplet CLK). Here, FIG. 6Bshows the microparticles A1 and A2 which flow through the flow path ofthe microchip 2 and are detected by the detection unit 3. Furthermore,FIG. 6C shows the droplets D1 and D2 which respectively contain themicroparticles A1 and A2.

In the example shown in FIGS. 6A to 6C, even if the microparticles A1and A2 are contained in the same droplet clock, the phase only changedby (ϕ2−ϕ1) (refer to FIG. 6B). Therefore, in a case in which themicroparticles A1 and A2 are contained in different droplets D1 and D2,when the delay time is set to the provisional delay time, there arecases in which the timing of the charge application to the desireddroplet deviates (refer to FIG. 6C). Therefore, in order to moreaccurately determine the timing when to apply the charge to thedroplets, it is necessary to adjust the delay time to an interval(hereinafter referred to as the phase) shorter than the droplet clock.

FIGS. 7A to 7C show images that are imaged by the droplet camera 4 atdifferent phases in relation to the same droplet clock. Morespecifically, FIGS. 7A to 7C show images of the microparticles that areimaged by the droplet camera 4 at each phase (FIG. 7A PHY=0, FIG. 7BPHY=90, FIG. 7C PHY=270) in relation to the same droplet clock. Amongthe phases where the time of the same droplet clock is divided into 360phases, FIGS. 7A to 7C respectively show the images in relation to the0th phase (FIG. 7A PHY=0), the 90th phase (FIG. 7B PHY=90), and the270th phase (FIG. 7C PHY=270) in time series order.

As illustrated with reference to FIGS. 6A to 6C, since there are casesin which different droplets D1 and D2 contain the microparticles A1 andA2 within the same droplet clock, in the example shown in FIG. 7A, it isalso possible to detect the bright spots B in the comparison region(Section 0) adjacent to the standard region (Section 1) using thecontrol unit 7. In other words, the control unit 7 discriminates thatthere are microparticles within the comparison region (Section 0). Inaddition, in the example shown in FIG. 7C, bright spots B are alsodetected in the other comparison region (Section 2) adjacent to thestandard region (Section 1). In other words, the control unit 7discriminates that there are microparticles within the comparison region(Section 2).

In the present step S₆, this makes it possible for the control unit 7 todiscriminate the phase deviation (the delay phase), to update theprovisional delay time, and to determine the delay time. Morespecifically, the control unit 7 updates the provisional delay time anddetermines the delay time by referring to the adjacent informationrelating to the number of the bright spots B of one of the comparisonregions of the two comparison regions that are adjacent to the standardregion in relation to the discharge direction of the droplets D in theimage information. Detailed description will be given below of the firstdelay time determination method and the second delay time determinationmethod as specific determination methods of the delay time withreference to FIG. 8 to FIG. 11.

Furthermore, here, description is given of an example where the brightspots B are detected in two regions (the two regions of “Section 1” and“Section 0” or “Section 2”), however, even in a case in which the brightspots are detected in the three regions of “Section 1”, “Section 0”, and“Section 2”, it is possible to determine the delay time in the samemanner as in the first delay time determination method or the seconddelay time determination method described below.

2-6-1 First Delay Time Determination Method

First, description will be given of the first delay time determinationmethod with reference to FIG. 8 and FIG. 9, in which the delay time isdetermined by referring to the bright spots of only “Section 2 (S2)” ofthe two comparison regions. FIG. 8 and FIG. 9 are graphs in which thehorizontal axis is the phase value, and the vertical axis is the numberof bright spots of the image of the droplets imaged by the dropletcamera 4 of the flow cytometer 1. More specifically, in the graphs, asshown in FIG. 8, the number of bright spots in the three regions of“Section 0 (S0)”, “Section 1 (S1)”, and “Section 2 (S2)” is shown.

First, in relation to “Section 1 (S1)”, the control unit 7 calculatesthe maximum value of the number of bright spots in “Section 2 (S2)” ofthe two comparison regions, which is positioned at the positivedirection side of the discharge direction of the droplets (refer to FIG.9, (1 a)).

Next, the control unit 7 calculates the time at which the number ofbright spots reaches 10% of the maximum value described above in theprocess where the number of bright spots increases toward a maximum in“Section 2 (S2)” (refer to point (1 b) and point (1 c) in FIG. 9).

Finally, the control unit 7 sets the time at which the number of brightspots reaches 10% of the maximum value as time at which the maximumnumber of bright spots is reached within the standard region, anddetermines the delay time. In other words, the control unit 7 calculatesthe phase value in relation to the point (1 b) and the point (1 c) anddetermines the delay time based on the phase value.

According to the above, in the flow cytometer 1, the control unit 7calculates the delay time by referring to the adjacent informationrelating to the number of the bright spots in the comparison regionpositioned in the positive direction side of the discharge direction ofthe droplets of the two comparison regions that are adjacent to thestandard region in relation to the discharge direction of the droplet Din the image information. In this manner, in the flow cytometer 1, it ispossible to accurately and automatically apply the charge to thedroplets without concerns that phase shifting will occur between thesynchronized droplets and the charge signal.

Furthermore, the first delay time determination method, which is anexample of the present step S₆, is used favorably in a case in which thedetection of the microparticles contained in the sample is performed inthe microchip 2 and the application of charge to the droplet D isperformed in air. In other words, the speed of the sample varies betweeninside the microchip 2 and in the air, and therefore in a case in whichit is necessary to adjust the timing at which the charge is applied tothe sample, it is particularly effective to determine the delay timeusing the first delay time determination method.

In addition, description is given of a case in which, in the first delaytime determination method, the control unit 7 calculates the phase valuebased on the value at which the number of bright spots reaches 10% ofthe maximum in the comparison region, however, the present technology isnot limited to this example. For example, it is also possible for thecontrol unit 7 to determine an appropriate delay time by calculating X %(where X is, for example, an arbitrary value such as 5 or 20) of themaximum number of bright spots.

2-6-2 Second Delay Time Determination Method

Next, description will be given of the second delay time determinationmethod with reference to FIG. 10 and FIG. 11, in which the delay time isdetermined by referring to the bright spots of the two comparisonregions Section 0 (S0) and Section 2 (S2). FIG. 10 and FIG. 11 aregraphs in which the horizontal axis is the phase value, and the verticalaxis is the number of bright spots of the image of the droplets imagedby the droplet camera 4 of the flow cytometer 1. More specifically, inthe graphs, as shown in FIG. 10, the number of bright spots in the threeregions of Section 0 (S0), Section 1 (S1), and Section 2 (S2) is shown.

First, in relation to Section 1 (S1), the control unit 7 calculates theaverage value of the maximum value and the minimum value of the numberof bright spots in Section 0 (S0) of the two comparison regions, whichis positioned at the negative direction side of the discharge directionof the droplets (refer to FIG. 11, (2 a)). In other words, as shown inFIG. 11, when the minimum value is 0, the control unit 7 calculates thevalue of the maximum value of the number of bright spots in Section 0(S0) multiplied by ½ (hereinafter referred to as the median value inSection 0).

Next, in the same manner as the case of Section 0 (S0), in relation toSection 1 (S1), the control unit 7 calculates the average value of themaximum value and the minimum value of the number of bright spots inSection 2 (S2) of the two comparison regions, which is positioned at thepositive direction side of the discharge direction of the droplets(refer to FIG. 11, (2 b)). In other words, as shown in FIG. 11, when theminimum value is 0, the control unit 7 calculates the value of themaximum value of the number of bright spots in Section 2 (S2) multipliedby ½ (hereinafter referred to as the median value in Section 2).

Next, the control unit 7 calculates a first time at which the ½ of themaximum number of bright spots is reached in the process of the numberof bright spots decreasing from the maximum in Section 0 (S0). In otherwords, the control unit 7 takes the median value in the Section 0,calculates a parallel straight line on the horizontal axis of the graph,and calculates the phase value of the point at which the straight linecrosses the point at which Section 0 (S0) is plotted (refer to point (2c) in FIG. 11).

Next, the control unit 7 calculates a second time at which the ½ of themaximum number of bright spots is reached in the process of the numberof bright spots increasing toward the maximum in Section 2 (S2). Inother words, in the same manner as in the case of Section 0 (S0), thecontrol unit 7 takes the median value in the Section 0, calculates aparallel straight line on the horizontal axis of the graph, andcalculates the phase value of the point at which the straight linecrosses the point at which Section 0 (S0) is plotted (refer to point (2d) in FIG. 11).

Finally, based on the first time and the second time, the control unit 7determines the delay time as (first time+second time)/2. In other words,the control unit 7 calculates the median point (refer to point (2 e) inFIG. 11) of the phase value in relation to the point (2 c) and the point(2 d) and determines the delay time with the phase value of the medianpoint set as the maximum number of bright spots within the standardregion.

According to the above, in the flow cytometer 1, the control unit 7calculates the delay time by referring to the adjacent informationrelating to the number of the bright spots in the two comparison regionsthat are adjacent to the standard region in relation to the dischargedirection of the droplets in the image information. In this manner, inthe flow cytometer 1, it is possible to accurately and automaticallyapply the charge to the droplets without concerns that phase shiftingwill occur between the synchronized droplets and the charge signal.

Furthermore, the second delay time determination method, which is anexample of the present step S₆, is used favorably in a case in which thesample is in the air from the position at which the microparticlescontained in the sample are detected until the position (the point atwhich the values of S0 and S2 are calculated) at which the applicationof a charge to the microparticles is performed.

In addition, description is given of a case in which, in the seconddelay time determination method, the control unit 7 calculates the phasevalue with the value at which the number of bright spots reaches ½ ofthe maximum in the comparison region as the median value, however, thepresent technology is not limited to this example. For example, it isalso possible for the control unit 7 to determine the delay time bycalculating the median value as 1/Y (where Y is, for example, anarbitrary value such as 2, 3, or 4) of the value of X % (where X is, forexample, an arbitrary value such as 90 or 80) of the maximum number ofbright spots in the comparison region.

2-7 Microparticle Sorting Step S₇

FIG. 12 is flow chart for illustrating step S₇ in which microparticlessuch as cells are sorted in the flow cytometer 1. In the microparticlesorting step S₇, the flow cytometer 1 performs sorting of the dropletscontaining microparticles such as cells based on the delay time which isdetermined in steps S₁ to S₆ described above. A trajectory directiondiscrimination step includes the processes of “the microparticledetection step S₇₁” and “the droplet discharge and charge applicationstep S₇₂”. Description will be given of each process below.

2-7-1 Microparticle Detection Step S₇₁

First, in the present step S₇₁, the detection unit 3 detects themicroparticles. The detection method may be performed in the same manneras the process in step S₁ described above.

2-7-2 Droplet Discharge and Charge Application Step S₇₂

Next, in the present step S₇₂, once the determined delay time haselapsed from the time at which the microparticles such as cells aredetected by the detection unit 3, the control unit 7 outputs a signal tothe charge unit 11 for performing charge application on the dropletscontaining the microparticles (refer to FIG. 12). Furthermore, thecharge unit 11 applies a charge to the droplets.

In this manner, in the flow cytometer 1, it is possible to accuratelyand automatically apply a charge to the desired microparticles such ascells. In addition, in the flow cytometer 1, it is possible to apply acharge in a state in which the delay time has been determined.Therefore, by irradiating the droplet D using the laser L2 only once theprovisional delay time has elapsed after the microparticles such ascells are detected by the detection unit 3, it is possible to accuratelyconfirm that the droplet D contains the microparticles while reducingthe usage amount of the laser.

The microparticle sorting apparatus and the delay time determinationmethod according to the present technology may also adopt the followingconfigurations.

(1) A microparticle sorting apparatus including a detection unit whichdetects microparticles flowing through a flow path; an imaging devicewhich images droplets containing the microparticles which are dischargedfrom an orifice provided on an edge portion of the flow path; a chargeunit which applies a charge to the droplets; and a control unit whichdetermines a delay time as from a time that the microparticles aredetected by the detection unit to the time at which the number of brightspots in a standard region, which is set beforehand, of imageinformation imaged by the imaging device reaches the maximum, making itpossible for the charge unit to apply a charge to the microparticlesonce the delay time has lapsed after the microparticles are detected bythe detection unit.

(2) The microparticle sorting apparatus according to (1), in which theimaging device images images of a plurality of the droplets at aplurality of different times, and in which the control unitpreliminarily determines the delay time using the time from when themicroparticles are detected by the detection unit to the time at whichthe number of bright spots within the standard region, which iscalculated by comparing the image information of the plurality ofdroplets imaged by the imaging device, reaches a maximum as aprovisional delay time.

(3) The microparticle sorting apparatus according to (2), in which theimaging device images the images of the plurality of droplets within ashorter time than a discharge interval time of each of the droplets oncethe provisional delay time has elapsed from the time at which themicroparticles are detected by the detection unit, and in which thecontrol unit updates the provisional delay time and determines the delaytime by referring to adjacent information relating to the number of thebright spots in at least one of two comparison regions that are adjacentto the standard region in relation to a discharge direction of thedroplets in the image information.

(4) The microparticle sorting apparatus according to (3), in which, ofthe two comparison regions, a first comparison region and a secondcomparison region are arranged in order in a discharge direction of thedroplets, and in which the control unit calculates the time at which thenumber of bright spots reaches a predetermined percentage of the maximumvalue in the second comparison region in a process where the number ofbright spots increases toward the time at which the number of brightspots reaches the maximum in the second comparison region, anddetermines the time to be the time at which the number of bright spotswithin the standard region reaches the maximum.

(5) The microparticle sorting apparatus according to (3) or (4), inwhich, of the two comparison regions, a first comparison region and asecond comparison region are arranged in order in a discharge directionof the droplets, in which the control unit calculates the first time atwhich the number of bright spots reaches a predetermined percentage ofthe maximum value in the first comparison region in a process where thenumber of bright spots decreases from the time at which the number ofbright spots reaches the maximum in the first comparison region,calculates the second time at which the number of bright spots reaches apredetermined percentage of the maximum value in the second comparisonregion in a process where the number of bright spots increases towardthe time at which the number of bright spots reaches the maximum in thesecond comparison region, and determines the time in which the number ofbright spots within the standard region reaches the maximum as (thefirst time+the second time)/2.

(6) The microparticle sorting apparatus according to any one of (1) to(5), in which the control unit adjusts a discharge frequency of thedroplets, and determines the optimal discharge frequency of the dropletsto be the discharge frequency at which the break-off point, where thedroplets start forming in the discharge direction of the droplets, isclosest to the orifice.

(7) The microparticle sorting apparatus according to any one of (1) to(6), further including a pair of deflection plates disposed opposite oneanother to interpose the droplets imaged by the imaging device, whichchange a progression direction of the droplets using an electrical forcewhich acts between the deflection plates and the charge.

(8) The microparticle sorting apparatus according to any one of (1) to(7), in which the microparticle sorting apparatus is a microchip-typeflow cytometer in which the orifice and the flow path are provided inthe microchip.

(9) A delay time determination method in a microparticle sorting deviceincluding causing a microparticle sorting device to detectmicroparticles flowing through a flow path; causing a microparticlesorting device to image a droplet containing the microparticles which isdischarged from an orifice provided on an edge portion of the flow path;and causing a microparticle sorting device to determine a delay time asfrom a time that the microparticles are detected until the time at whicha number of bright spots in a standard region, which is set beforehand,of image information of droplets that are imaged reaches the maximum.

(10) The delay time determination method according to (9), furtherincluding imaging the images of a plurality of the droplets at aplurality of times; and preliminarily determining the delay time usingthe period of from the time at which the microparticles are detecteduntil the time at which the number of bright spots within the standardregion, which is calculated by comparing the image information of theplurality of droplets which are imaged, reaches the maximum as theprovisional delay time.

(11) The delay time determination method according to (10), furtherincluding imaging the images of the plurality of droplets within ashorter time than a discharge interval time of each of the droplets oncethe provisional delay time has elapsed from the time at which themicroparticles are detected; and updating the provisional delay time anddetermining the delay time by referring to adjacent information relatingto the number of the bright spots in at least one of two comparisonregions that are adjacent to the standard region in relation to adischarge direction of the droplets in the image information.

(12) The delay time determination method according to (10), in which, ofthe two comparison regions, a first comparison region and a secondcomparison region are arranged in order in a discharge direction of thedroplets, the method further including calculating the time at which thenumber of bright spots reaches a predetermined percentage of the maximumvalue in the second comparison region in a process where the number ofbright spots increases toward the time at which the number of brightspots reaches the maximum in the second comparison region; anddetermining the time to be the time at which the number of bright spotswithin the standard region reaches the maximum.

(13) The delay time determination method according to (11) or (12), inwhich of the two comparison regions, a first comparison region and asecond comparison region are arranged in order in a discharge directionof the droplets, the method further including calculating the first timeat which the number of bright spots reaches a predetermined percentageof the maximum value in the first comparison region in a process wherethe number of bright spots decreases from the time at which the numberof bright spots reaches the maximum in the first comparison region;calculating the second time at which the number of bright spots reachesa predetermined percentage of the maximum value in the second comparisonregion in a process where the number of bright spots increases towardthe time at which the number of bright spots reaches the maximum in thesecond comparison region; and determining the time in which the numberof bright spots within the standard region reaches a maximum as (thefirst time+the second time)/2.

The present disclosure contains subject matter related to that disclosedin Japanese Priority Patent Application JP 2012-080192 filed in theJapan Patent Office on Mar. 30, 2012, the entire contents of which arehereby incorporated by reference.

It should be understood by those skilled in the art that variousmodifications, combinations, sub-combinations and alterations may occurdepending on design requirements and other factors insofar as they arewithin the scope of the appended claims or the equivalents thereof.

What is claimed is:
 1. A microparticle sorting apparatus comprising: adetector configured to detect microparticles flowing through a flowpath; an imaging device configured to obtain a plurality of images of adroplet containing at least one of the microparticles, wherein thedroplet is discharged from an orifice provided on an edge portion of theflow path; and a controller configured to: control the imaging device toobtain the plurality of images, the plurality of images having a commonregion in respective images of the plurality of images; and determine adelay phase by comparing a brightness characteristic within the commonregion between a first image of the plurality of images and at least onesecond image of the plurality of images, wherein the brightnesscharacteristic is indicative of an amount of pixels having a brightnesshigher than a threshold.
 2. The microparticle sorting apparatus of claim1, wherein the controller is further configured to set a timing forapplying charge to droplets based on the delay phase.
 3. Themicroparticle sorting apparatus of claim 1, wherein the controller isfurther configured to determine the delay phase by comparing abrightness characteristic in at least one comparison region to abrightness characteristic in the common region for an image of theplurality of images, wherein the at least one comparison region isadjacent to the common region along a discharge direction of thedroplets in the image.
 4. The microparticle sorting apparatus of claim3, wherein the at least one comparison region includes a firstcomparison region and a second comparison region arranged on oppositesides of the common region, and wherein the controller is configured todetermine the delay phase based on a brightness characteristic in thefirst comparison region and a brightness characteristic in the secondcomparison region.
 5. The microparticle sorting apparatus of claim 4,wherein the controller is further configured to: calculate a first timeat which a brightness characteristic of the first comparison regionreaches a predetermined percentage of a maximum value; calculate asecond time at which a brightness characteristic of the secondcomparison region reaches a predetermined percentage of the maximumvalue; and determine a time for the common region as (the first time+thesecond time)/2.
 6. The microparticle sorting apparatus of claim 4,wherein the controller is further configured to adjust a dischargefrequency of the droplets based on the delay phase, and determine anoptimal discharge frequency of the droplets to be the dischargefrequency at which a point where the droplets are formed is proximatethe orifice.
 7. The microparticle sorting apparatus of claim 1, furthercomprising: a pair of deflection plates disposed opposite one another tointerpose the droplets imaged by the imaging device, which change aprogression direction of the droplets using an electrical force whichacts between the deflection plates and a charge applied to the droplets.8. The microparticle sorting apparatus of claim 1, wherein themicroparticle sorting apparatus is a microchip-type flow cytometer inwhich the orifice and the flow path are provided in a microchip.
 9. Themicroparticle sorting apparatus of claim 1, wherein the controller isfurther configured to control the imaging device to obtain the pluralityof images based on a time when one of the microparticles is detected bythe detector.
 10. A delay phase determination method comprising:detecting, using a microparticle sorting device, microparticles flowingthrough a flow path; obtaining, using the microparticle sorting device,a plurality of images of a droplet including at least one of themicroparticles, the plurality of images having a common region inrespective images of the plurality of images; and determining a delayphase by comparing a brightness characteristic within the common regionbetween a first image of the plurality of images and at least one secondimage of the plurality of images, wherein the brightness characteristicis indicative of an amount of pixels having a brightness higher than athreshold.
 11. The delay phase determination method of claim 10, themethod further comprising setting a timing for applying charge todroplets based on the delay phase.
 12. The delay phase determinationmethod of claim 10, wherein determining the delay phase furthercomprises comparing a brightness characteristic in at least onecomparison region to a brightness characteristic in the common regionfor an image of the plurality of images, wherein the at least onecomparison region is adjacent to the common region along a dischargedirection of the droplets in the image.
 13. The delay phasedetermination method of claim 12, wherein the at least one comparisonregion includes a first comparison region and a second comparison regionarranged on opposite sides of the common region, and determining thedelay phase further comprises determining the delay phase based on abrightness characteristic in the first comparison region and abrightness characteristic in the second comparison region.
 14. The delayphase determination method of claim 13, the method further comprising:calculating a first time at which a brightness characteristic of thefirst comparison region reaches a predetermined percentage of a maximumvalue; calculating a second time at which a brightness characteristic ofthe second comparison region reaches a predetermined percentage of themaximum value; and determining a time for the common region as (thefirst time+the second time)/2.
 15. The delay phase determination methodof claim 13, the method further comprising: adjusting a dischargefrequency of the droplets based on the delay phase; and determining anoptimal discharge frequency of the droplets to be the dischargefrequency at which a point where the droplets are formed is proximate tothe orifice.
 16. The delay phase determination method of claim 10,wherein obtaining the plurality of images further comprises obtainingthe plurality of images based on a time when one of the microparticlesis detected by the microparticle sorting device.
 17. The microparticlesorting apparatus of claim 1, wherein the brightness characteristic isrepresentative of one or more microparticles contained within one ormore droplets.
 18. The microparticle sorting apparatus of claim 1,wherein the delay phase corresponds to a time relative to a dischargetime interval between droplets.
 19. The microparticle sorting apparatusof claim 1, wherein the droplet is in the common region for at least oneof the plurality of images and the droplet is not in the common regionfor at least one of the plurality of images.
 20. The microparticlesorting apparatus of claim 1, wherein the common region is at the samesection along a discharge direction of the droplet.